Levels of genetic variability and differentiation within and among populations of the ring-necked pheasant (Phasianus colchicus) are described based on allozyme data from 26 electrophoretic loci. Mean individual heterozygosity was high (.hivin.H = 0.087). Spatial analysis revealed significant heterogeneity among pheasants sampled from 10 playa basins in the Texas Panhandle (mean Fst = 0.086; .chi.2 = 166.27, P < 0.05). Heterogeneity of interpopulational spatial relationships was observed across separate portions of the study area. The high degree of spatial differentiation could be explained as a result of limited dispersal accentuated by heterogeneous point stockings.