LOCALIZATION AND PROPERTIES OF PHOSPHOPROTEIN PHOSPHATASE IN THE FROG EGG AND EMBRYO

Open Access
Publisher Website
Google Scholar
Abstract
Phosphoprotein phosphatase (PPP ase) activity of various centrifugal fractions and extracts of homogenates of Rana pipiens ovaries and embryos was studied in the presence and in the absence of added phosphoprotein substrates, and as a function of incubation time, pH, temperature and ionic concentrations. The pigment-containing centrifugal fraction was found to have the highest PPPase activity when ovarian fractions were incubated in the presence of added phosphoprotein. The enzyme activity of the pigment-containing fraction was found to drop precipitously after fertilization, slowly increase from gastrulation to neurulation, and rise to a maximum at tailbud stage. The activity declined after hatching.