Tissue Culture Assay for Moloney Leukemia Virus2
- 1 February 1970
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 44 (2) , 403-412
- https://doi.org/10.1093/jnci/44.2.403
Abstract
The JLS-V9 bone marrow cell line was infected with Moloney leukemia virus (MLV). A new surface antigen was detected on the infected cells by indirect membrane immunofluorescence and immune adherence to living target cells. The serum of MLV-immunized mice was used as the reagent. The time course of antigen expression was studied with various sources and dilutions of the virus. A virus preparation was also compared in tests for leukemogenic and immunogenic activity in vivo and antigen-inducing ability in vitro. The tissue culture test provided a useful tool to assess MLV infectivity.Keywords
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