QUANTITATIVE IN VITRO STUDIES ON STIMULATION OF MURINE HAEMOPOIETIC CELLS BY COLONY STIMULATING FACTOR

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Abstract
A new standardization method for Colony Stimulating Factor (CSF) is described and criteria are introduced which enables stimulating activity to be assessed independent of absolute colony numbers. With this method CSF preparations from different sources are compared and evidence is presented that suggests an identical mechanism of action for these substances. Data are presented on the increase in colony numbers that is induced by the addition of erythrocyte lysates to the cultures. The relationship between colony forming cells that are stimulated by CSF alone and cells stimulated by the combined action of CSF and erythrocyte lysate is discussed.