Stability of polysome-associated polyadenylated RNA from soybean suspension culture cells
- 20 March 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (6) , 1013-1018
- https://doi.org/10.1021/bi00573a013
Abstract
The half-life of polysome-associated, poly(A)-RNA is exponentially growing soybean (Glycine max) suspension culture cells was determined with pulse-chase experiments. Based on a best fit from a computer analysis of the data, 2 decay components for poly(A)-RNA were found. One component had a half-life of approximately 0.6 h; the other about 30 h, similar to the doubling time of the cultures. At the beginning of the chase period, the short-lived component represented approximately 90% of the total poly(A)-RNA in the polysomes. This percentage decreased with time so that, under steady-state conditions, the long-lived component probably represented the majority of poly(A)-RNA.This publication has 8 references indexed in Scilit:
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