Production of Starch-like Material From Glucose-1-phosphate by Diphtheria Bacilli

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Abstract
The bacteria tested included 17 strains of Corynebacterium diphtheriae, 12 strains of streptococci Lancefield, groups A to F, 9 strains of streptococci from endocarditis which form dextran from sucrose, and other spp.; for comparison, 3 strains of Neisseria perflava were included. The bacteria were inoculated into 3 media containing 2% glucose-1-phosphate, 2% glucose, or 2% sucrose. After incubation at 37 [degree]C for 7 days, cultures with good growth were centrifuged and the supernatant fluids and bacterial sediments were tested for starch-like material by adding a soln. containing 0.02% I2 and 0.2% KI. All strains of C. diphtheriae reacted positively for starch when grown in the medium containing glucose-1-phosphate. Some of the streptococci gave the same response. Except for N. perflava, none of the other bacteria tested produced any detectable amt. of iodine-coloring material.

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