Mrc1 channels the DNA replication arrest signal to checkpoint kinase Cds1
- 8 October 2001
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 3 (11) , 966-972
- https://doi.org/10.1038/ncb1101-966
Abstract
Checkpoint responses change as cells proceed through the cell cycle. Here we describe a novel checkpoint gene in fission yeast, mrc1 (mediator of replication checkpoint), that confers activation of the checkpoint kinase Cds1 to DNA synthesis (S) phase. Mrc1 associates with Cds1 and is required for regulation of Cds1 by the checkpoint kinase Rad3. Mrc1 is regulated by the cell cycle, with the appearance of Mrc1 mRNA and protein coinciding with S phase. We propose that coordinated expression of Mrc1 with replication control proteins helps to ensure activation of the appropriate checkpoint response during DNA replication.Keywords
This publication has 43 references indexed in Scilit:
- [56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombePublished by Elsevier ,2004
- Threonine-11, Phosphorylated by Rad3 and ATM In Vitro, Is Required for Activation of Fission Yeast Checkpoint Kinase Cds1Molecular and Cellular Biology, 2001
- Roles of the Mitotic Inhibitors Wee1 and Mik1 in the G2 DNA Damage and Replication CheckpointsMolecular and Cellular Biology, 2001
- Partners and pathwaysTrends in Genetics, 2000
- DNA Damage-Induced Activation of p53 by the Checkpoint Kinase Chk2Science, 2000
- Cdc25 Mitotic Inducer Targeted by Chk1 DNA Damage Checkpoint KinaseScience, 1997
- Cdc2 tyrosine phosphorylation is required for the DNA damage checkpoint in fission yeast.Genes & Development, 1997
- rad -Dependent Response of the chk1 -Encoded Protein Kinase at the DNA Damage CheckpointScience, 1996
- A kinase from fission yeast responsible for blocking mitosis in S phaseNature, 1995
- The fission yeast cdc18+ gene product couples S phase to START and mitosisCell, 1993