Primary monolayer culture of liver parenchymal cells and kidney cortical tubules as a useful new model for biochemical pharmacology and experimental toxicology

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Abstract
Freshly isolated liver parenchymal cells were maintained in either shortterm monolayer, suspension or long-term monolayer culture. Rapidly occurring processes through hepatocellular membrane, e.g., the enhanced amino acid transport and the concomitantly increased potassium influx following progressive starvation, were kinetically evaluated best in short-term monolayer culture. The inducibility of tyrosine aminotransferase by glucagon, dexamethasone, and a combination of both was compared in suspension and in monolayer culture. The induction of slowly inducible foreign compound-metabolizing enzymes, (e.g., ethoxycoumarin-O-dealkylase, p-nitroanisole-O-demethylase, and UDP-glucuronyltransferase) by phenobarbital, 3-methylcholanthrene, and dexamethasone were studied in long-term monolayer culture. The latter system was also used to maintain isolated kidney cortical tubules for the investigation of renal enzyme adaptation during progressive time in culture.