Distribution and effect of some polychlorinated biphenyls in the hemopoietic tissues

Abstract
Distribution of 14C-labeled 2,4'',5-trichlorobiphenyl, 2,2'',4,4'',5,5''-hexachlorobiphenyl and 2,2'',3,3'',4,4'',5,6''-octachlorobiphenyl was studied in the hematopoietic tissues of squirrel monkey and mice (C67 Bl) using whole-body autoradiography. An accumulation of radioactivity was seen in the bone marrow of monkey 24 and 48 h after i.v. injection of hexachlorobiphenyl and trichlorobiphenyl, respectively. High concentration of radioactivity was also observed in the bone marrow of normal mice injected i.v. with octachlorobiphenyl. A combination of whole-body autoradiography and spleen-colony assay in supralethally irradiated mice implanted with syngeneic bone marrow cells indicated the major part of radioactivity to be localized outside the bone marrow hemic compartment, probably in the fat. No effect of hexachlorobiphenyl but a significant inhibition with octachlorobiphenyl (10-5 M) and especially trichlorobiphenyl (10-5 M) was seen on the formation of granulocytic colonies from mouse progenitor cells in vitro.

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