A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP‐dependent RNA helicase

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Abstract
The spp81/ded1 mutations were isolated as suppressors of a Saccharomyces cerevisiae pre‐mRNA splicing mutation, prp8‐1, The SPP81/DED1 gene encodes a isolated plasmids which were able to suppress the cold‐sensitive growth defect of SPP81 mutants. These plasmids encoded a gene (named DBP1) which mapped to chromosome XVI and not to the SPP81/DED1 locus on chromosome XV. The cloned gene suppressed the defect of SPP81/ded1 mutants when present on both high and low copy‐number plasmids but complemented SPP81/ded1 null mutants only when present on high copy‐number plasmids. In contrast to the SPP81/DED1 gene the DBP1 gene was not essential for cell viability. The nucleotide sequence of the DBP1 gene revealed that it also encoded a putative ATP‐dependent RNA helicase which showed considerable similarity at the amino acid level to the SPP81/DED1 protein.