Mycoplasma pneumoniae DNA gyrase genes

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Abstract
We have identified a clone from a .lambda. EMBL3 library containing a 19 kb insert of Mycoplasma pneumoniae DNA which includes the genes that encode both subunits of DNA gyrase. The gyrB gene and the 5'' end of the gyrA gene have been subcloned into M13. The gyrB gene is 1953 bp in length and overlaps the gyrA gene by a single base. The nucleotide sequence of these subclones has significant homology to previously reported gyrase genes. In terms of the size of the gyrB gene and its proximity to the gyrA gene, M. pneumoniae is more similar to Bacillus subtilis than to Escherichia coli.