Selective interference with viral RNA formation in vitro by specific inhibition with synthetic polyribonucleotides.
- 1 October 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 56 (4) , 1333-1338
- https://doi.org/10.1073/pnas.56.4.1333
Abstract
Previous experiments have established that RNA replicases induced by RNA viruses require homologous and intact RNA for proper synthetic activity. studies of the Q[beta]-replicase suggested that this enzyme recognizes a secondary structure formed by the pairing of two complementary sequences, initial and terminal, one containing predominantly A and the other U. In conformity with this model, it has been found that Q[beta]-repllcase is specifically inhibited by synthetic polynucleotides composed principally of either A or U. Other polynucleotides, contain-ing mainly or solely C or G, are Inert. It was further shown that prior attachment of homologous template to enzyme eliminates the immediate inhibition by either poly A or U. The discovery of specific template requirements and the studies reported here suggest a new approach in the search for a novel and highly selective interference with viral replication via compounds which can neutralize the recognition mechanism between a replicase and its homologous template.This publication has 9 references indexed in Scilit:
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