Regional mapping of the gene coding for enolase-2 on human chromosome 12

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Abstract
Enolase-2 (ENO2), previously termed 14-3-2 protein, is an isozyme of enolase that is enriched in neuronal tissue. The gene coding for ENO2 was previously assigned to human chromosome 12. The present study presents data for a regional mapping of gene ENO2 using cell hybrids containing various deletions of human chromosome 12. These deletions were produced by treatment with chromosome-breaking agents. Cytogenetic analysis has allowed assignment of ENO2 to the short arm of chromosome 12, in the region of pter-pizos. This assignment is consistent with the segregation pattern of the 93 hybrid clones analysed. The segregation pattern has also established the linear order of 6 genes on chromosome 12: pter — TPI — GAPD — LDHB — ENO2— centromere — SHMT — PEPB — qter. Estimation of the relative distances between the 6 genes on chromosome 12 has been made by a statistical mapping analysis of the segregation data of the hybrid clones. A set of deletion hybrids containing various combinations of these 6 markers has been established for a rapid regional mapping of genes in one of these regions on chromosome 12.