Collapse of ATP-Induced pH Gradient by Sodium Ions in Microsomal Membrane Vesicles Prepared from Atriplex gmelini Leaves
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 89 (1) , 180-183
- https://doi.org/10.1104/pp.89.1.180
Abstract
Sealed microsomal membrane vesicles were prepared from leaves of a 250 millimolar NaCl-grown halophyte (Atriplex gmelini C.A. Mey). The vesicles exhibited ATP-dependent proton-transporting activity which was inhibited 60% by NO3- (50 millimolar) but not by vanadate (100 micromolar) and 23% by oligomycin (10 micrograms per milliliter), suggesting that tonoplast-derived vesicles were the major constituents of the preparation. The pH gradient established by the vesicles by ATP in the presence of oligomycin collapsed upon the addition of Na+ salts. The vesicles took up Na+ ions in the presence of ATP and this activity was canceled by gramicidin. These results suggest that Na+ ions were taken up by the vesicles via a Na+-specific uptake system, possibly a Na+/H+ antiport.This publication has 9 references indexed in Scilit:
- Sodium, Potassium, Chloride, and Betaine Concentrations in Isolated Vacuoles from Salt-Grown Atriplex gmelini LeavesPlant Physiology, 1987
- Membrane Transport in Isolated Vesicles from Sugarbeet TaprootPlant Physiology, 1987
- The function of tonoplast ATPase in intact vacuoles of red beet is governed by direct and indirect ion effectsPlanta, 1985
- Na+/H+ Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgarisPlant Physiology, 1985
- Density Gradient Localization of Plasma Membrane and Tonoplast from Storage Tissue of Growing and Dormant Red BeetPlant Physiology, 1984
- Characterization of Passive Ion Transport in Plasma Membrane Vesicles of Oat RootsPlant Physiology, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Salt Responses of Enzymes from Species Differing in Salt TolerancePlant Physiology, 1972
- [10] Assay of inorganic phosphate, total phosphate and phosphatasesPublished by Elsevier ,1966