ELECTRON TRANSPORT PARTICLES FROM BACILLUS STEAROTHERMOPHILUS

Abstract
Downey, Ronald J. (University of Nebraska, Lincoln), Carl E. Georgi, and Walter E. Militzer . Electron transport particles from Bacillus stearothermophilus . J. Bacteriol. 83: 1140–1146. 1962—Electron-transport particles (ETP) have been isolated from Bacillus stearothermophilus . They are capable of oxidizing such substrates as succinate, malate, diphosphopyridine nucleotide, p -phenylenediamine, and hydroquinone. Difference spectra indicated bands of cytochromes a 3 , b , and c . A chromatographic procedure for purification of cytochrome c has been described. The role of quinonelike intermediates in the ETP was implied by the restorative effect of coenzyme Q and vitamin K 1 on material treated with lipid solvent. Isolation and identification of coenzyme Q from the thermophile indicated it was similar to coenzyme Q 10 of mammalian sources.

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