The content of some amino acids in young apple shoots in relation to frost resistance

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Abstract
In the years 1963–1965 the content of proline, asparagine, glutamic and γ-aminobutyric acids in young apple (Malus cultivars) shoots was studied. Two groups of apple varieties differing in frost resistance were included into experiments: the Yellow Transparent and Oberländer Himbeerapfel varieties as frost-resistant ones and-the Ontario and Reinette du Canada varieties as sensitive to frost. In the course of experiment γ-aminobutyric acid showed a relatively balanced level but glutamic acid, asparagine and especially proline were found in greater amount in the frost-resistant varieties. According to the results of this experiment the amount of proline—along with many other factors—can be considered as one of the indicators of the length of winter dormancy and also of the frost resistance of apple-trees at the beginning of the winter period.