Immunofluorescent examination of the nasal mucous membrane of mouse in prophylactic trials against Sendai virus.

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Abstract
Sendai virus infection was examined by the immunofluorescent method in the nasal mucous membrane (NMM) mice having received each of 5 kinds of propylactic treatment by direct or contact exposure. Administration of interferon or poly I:C via the nasal route decreased in number the viral-antigen-positive cells in NMM in the postexposure period, and the latter treatment protected the lower respiratory organs from infection. Immunoprophylaxis of the entire respiratory tract against infection was nearly complete with 6 doses of UV-inactivated vaccine via the nasal route, but not by administration of alginate adjuvant UV-vaccine via the percutaneous route or ether-inactivated vaccine via the nasal route. The adjuvant vaccine protected the lower respiratory tract against infection. Except for 1 successful UV-vaccine group, the test-positive cells in NMM of the 5 vaccinated groups and control increased markedly until postexposure day (PED) 5 and 10 by direct and contact infection, respectively, and thereafter decreased slowly. Viral antigen in the trachea and lungs disappeared on PED 7 and 12 with the development of serum antibody after infection by the 2 methods. This investigation of viral-antigen-positive cells in the entire respiratory tract, particularly in NMM differentiated in topical immunity and the viral infection between the 2 regions.

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