Propagation of Pathogenic Fungi in the Yolk Sac of Embryonated Eggs.

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Abstract
Actinomyces bovis. Nocardia asteroides. N. intracellularis, Sporntrichum schenckii. Histoplasma capsulatum. Cryptococcus neoformans and Coccidioides immitis have been readily propagated in the yolk /sac of developing chick embryos. This part of the embryonated egg provides environmental and nutritional conditions which are well suited to the requirements of these organisms. Embryos of 8-9 day incubation are inoculated into the yolk sac. The organisms listed are easily maintained in serial passage. The method offers a new approach to the study of infections caused by these agents and a means for assaying therapeutic agents, and has been found useful for the direct isolation and identification of pathogenic fungi from material derived from human cases.
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