COMPARISON AND OPTIMIZATION OF FILTER ELUTION METHODS FOR THE MEASUREMENT OF AIRBORNE ALLERGEN
Open Access
- 1 December 1992
- journal article
- Published by Oxford University Press (OUP) in Annals of Work Exposures and Health
- Vol. 36 (6) , 575-587
- https://doi.org/10.1093/annhyg/36.6.575
Abstract
We have examined the influence of filter type and elution method on the levels measured for an important occupational aeroallergen, rat urine. Three filter types [glass fibre (GF/A), and two forms of polytetrafluoroethylene (PTFEG and PTFES)] and three elution methods [syringe elution ([syr]), sonication ([sonic]) and test tube ([u]) elution] were studied. There were no significant differences in the rat urinary aeroallergen (RUA) concentrations recovered from GF/A and PTFEG when eluted in syringes or by sonication (GF/A only) in 0.1 M NH4HCO3 buffer. The allergen yield from GF/A and PTFEG increased nine- and three-fold, respectively (PPS[xyz] and PTFEG[syr], or from paired PTFES[syr] and PTFEG[syr] We recommend that 0.5% v/v Tween 20 is added to elution buffers. PTFES[u]was found to be the simplest, most sensitive and reproducible method (40–70% variability) for aeroallergen elution.Keywords
This publication has 0 references indexed in Scilit: