Potassium content and cell volume of centrifuged erythrocytes

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Abstract
The K content of plasma separated from rapidly sedimenting bloods either by simple settling or centrifuging (4500 or 10,000 r.p.m.) was practically constant; as human cells contain 25 times as much K as plasma, it follows that no K is expressed from cells by centrifuging. That water content is also constant was shown by washing out intercellular plasma from between settled and centrifuged batches of cells by resuspension in saline followed by recentrifuging. The protein in the supernatant saline was estimated colorimetrically and in this way a constant absolute volume of cells was obtained (settled or centrifuged volume minus intercellular plasma volume).

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