Crystal Structure of the Mammalian Grb2 Adaptor
- 14 April 1995
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 268 (5208) , 291-293
- https://doi.org/10.1126/science.7716522
Abstract
The mammalian growth factor receptor-binding protein Grb2 is an adaptor that mediates activation of guanine nucleotide exchange on Ras. Grb2 binds to the receptor through its SH2 domain and to the carboxyl-terminal domain of Son of sevenless through its two SH3 domains. It is thus a key element in the signal transduction pathway. The crystal structure of Grb2 was determined to 3.1 angstrom resolution. The asymmetric unit is composed of an embedded dimer. The interlaced junctions between the SH2 and SH3 domains bring the two adjacent faces of the SH3 domains in van der Waals contact but leave room for the binding of proline-rich peptides.Keywords
This publication has 27 references indexed in Scilit:
- The Crystal Structures of the SH2 Domain of p56lckComplexed with Two Phosphonopeptides Suggest a Gated Peptide Binding SiteJournal of Molecular Biology, 1995
- NMR structure of the N-terminal SH3 domain of GRB2 and its complex with a proline-rich peptide from SosNature Structural & Molecular Biology, 1994
- Structure of the N-terminal SH3 domain of GRB2 complexed with a peptide from the guanine nucleotide releasing factor SosNature Structural & Molecular Biology, 1994
- Solution structure and ligand–binding site of the carboxy–terminal SH3 domain of GRB2Structure, 1994
- Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domainsNature, 1994
- Orientation of Peptide Fragments from Sos Proteins Bound to the N-Terminal SH3 Domain of Grb2 Determined by NMR SpectroscopyBiochemistry, 1994
- The CCP4 suite: programs for protein crystallographyActa Crystallographica Section D-Biological Crystallography, 1994
- The crystal structure of human CskSH3: structural diversity near the RT‐Src and n‐Src loopFEBS Letters, 1994
- SH2 domains recognize specific phosphopeptide sequencesPublished by Elsevier ,1993
- Surface, subunit interfaces and interior of oligomeric proteinsJournal of Molecular Biology, 1988