Assessment of recombinant dog allergens Can f 1 and Can f 2 for the diagnosis of dog allergy
- 12 October 2004
- journal article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 34 (10) , 1576-1582
- https://doi.org/10.1111/j.1365-2222.2004.02071.x
Abstract
Summary: Background The use of recombinant allergens for the diagnosis and immunotherapy of allergy may offer several advantages over allergen extracts.Objective To produce recombinant dog allergens Can f 1 and Can f 2 in Pichia pastoris yeast and to assess their suitability for the diagnosis of dog allergy.Methods Clinically diagnosed dog‐allergic patients' and healthy non‐atopic dog owners' reactivities against recombinant Can f 1 and Can f 2 and commercial dog epithelial extract were studied by a panel of methods including skin prick test (SPT), ELISA and IgE immunoblotting.Results Recombinant Can f 1 and Can f 2 were found immunologically functional: they bound dog‐allergic patients' IgE in immunoblotting and inhibited specifically the binding of IgE to their natural counterparts in the dog allergen extract. Moreover, patients' IgE reactivity in immunoblotting to natural Can f 1 and their SPT with the recombinant allergen were perfectly concordant (φ coefficient 1.0, PPConclusions The recombinant allergens can be used reliably to identify Can f 1 and Can f 2‐sensitized individuals. However, the two allergens are insufficient as reagents for diagnosing dog allergy.Keywords
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