CYTOCHEMICAL LOCALIZATION OF TEN OXIDATIVE ENZYMES OF RAT ANTERIOR PITUITARY AND THYROID GLANDS DURING VARIOUS PHASES OF SECRETORY ACTIVITY

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Abstract
SUMMARY: The cytochemical distribution and changes of ten oxidative enzyme systems were studied in rat anterior pituitary and thyroid glands in various phases of secretory activity. Enzyme activity was always localized to discrete, small cytoplasmic particles thought to be mitochondria. Reduced diphosphopyridine nucleotide (DPNH)-Nitro-BT reductase activity in the thyroid gland was also found in nuclear membranes and in cytoplasmic clumps (probably endoplasmic reticulum). There was an apparent increase in number and size and migration toward the Golgi zone of these granules (mitochondria ?) in gonadotrophic cells of castrated rats. No apparent difference was observed in the thyroid gland following thyroxine treatment and exposure to cold.