Replication of PARA (Defective SV40)-Adenoviruses in Simian Cells

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Abstract
SUMMARY Human adenovirus types I, 2, 3, 5, 6, 7, 12, 14, I6, and 21 failed to repli- cate beyond input titres in green monkey kidney (GMK) cells. Co-infection of the monkey cells with papovavirus SV4o stimulated replication of all the adenoviruses tested. Addition of the defective SV4o genome in the PARA particle carried by an adenovirus type 7 population enabled all the adeno- viruses to replicate in the GMK cells. Growth kinetics of the adenoviruses in GMK cells co-infected with SV4o were similar to the kinetics of the virus following addition of PARA. A latent period of 16 to 24 hr was followed by an exponential increase in infectious virus between 24 and 48 hr after inocu- lation. Synthesis of the PAPA component closely paralleled that of the adenoviruses. Both the adenoviruses and PAPA remained largely cell- associated throughout the growth cycle. During the replication of the trans- capsidants, the titre of the adenovirus was always greater than the titre of the PAPA component. Ratios were generally in the order of 5oo infectious units of adenovirus per infectious unit of PAPA. These results differed from those obtained with the parent PARA-adenovirus type 7 population in which the adenovirus to PAPA ratio was approximately 3 : I.

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