Determination of fumarate and malate in animal tissues

Abstract
Fumarate is reduced to succinate in the presence of Zn and phosphoric acid and the succinate formed is detd. manometrically with succinic dehydrogenase. The cone, of 1([long dash])malate is calculated from the equilibrium constant. At pH 7.4 the ratio 1([long dash])malate/fumarate was 2.65 at 50[degree], 3.17 at 40[degree], 3.54 at 30[degree] and 4.57 at 20[degree].

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