Effect of Intrinsic Factor on Vit. B12 Uptake by Rat Intestine in vitro.

Publisher Website
Google Scholar
Abstract
Sacs of everted small intestine of the rat incubated in bicarbonate-saline containing glucose and B12 showed only a very small B12 uptake in the absence of a source of intrinsic factor. In the presence of neutralized rat gastric juice the stimulation of B12 uptake was 10- to 25-fold. No B12 appeared on the serosal side of the preparation. Stimulation of B12 uptake was most marked in segments of intestine taken from the mid-portion of the small intestine. Evidence is presented that neither B12 nor intrinsic factor when added alone are adsorbed to the intestinal wall but must be present simultaneously to give B12 uptake.