The ultra-violet absorption spectra of certain aromatic amino-acids, and of the serum proteins

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Abstract
Following the work of Dhere and others, the writer has measured the ultra-violet absorption spectra of exceptionally pure specimens of tyrosine, tryptophane, and phenyl-alanine, and of the serum proteins. Aromatic amino-acids: Tyrosine: Two new bands at wave lengths of of A 2240 and 1940 have been discovered. Tryptophane: Two bands have been observed, at wave lengths of A 2790 and 2180. Phenyl-alanine: One band only has been observed. Serum proteins: Six preparations of horse, and 6 of human protein were used; care was taken to exclude various possibilities of experimental error, e.g., by "scattering" of the radiation due to the colloidal condition of the proteins. The values obtained for the absorption spectra of horse and of human serum proteins correspond very closely; this result is contrary to Judd Lewis'' findings. From the figures thus obtained, a formula is given whereby the ratio of albumin to globulin, in a mixture of the two, may be calculated.

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