Mutational analysis of the putative PLA2-inhibiting sequence of annexin 1
- 1 November 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 293 (1-2) , 34-36
- https://doi.org/10.1016/0014-5793(91)81146-y
Abstract
Annexin 1 has been proposed to inhibit phospholipase A2 by direct interaction through a specific amino acid sequence spanning residues 246–254. The possible role of this region was investigated by protein engineering. Three point mutations and a deletion have been performed. The four mutant proteins have been expressed in E. coli, purified and tested for calcium and lipid binding, and for phospholipase inhibition. All mutant proteins conserved the properties of the wild-type recombinant protein. This result clearly demonstrates that this part of the molecule is not involved in the inhibition of phospholipase A2Keywords
This publication has 14 references indexed in Scilit:
- Protein-synthesis-dependent induction of annexin I by glucocorticoidBiochemical Journal, 1991
- Interaction of synthetic peptides from annexin I and uteroglobin with lipid monolayers and their effect on phospholipase A2 activityBiochemical Society Transactions, 1990
- Synthetic peptide from lipocortin I has no phospholipase A2 inhibitory activityFEBS Letters, 1989
- Synthesis of p36 and p35 is increased when U-937 cells differentiate in culture but expression is not inducible by glucocorticoids.Molecular and Cellular Biology, 1989
- Novel anti-inflammatory peptides from the region of highest similarity between uteroglobin and lipocortin INature, 1988
- Calcium-dependent phospholipid- (and membrane-) binding proteinsBiochemistry, 1988
- Two human 35 kd inhibitors of phospholipase A2 are related to substrates of pp60v-src and of the epidermal growth factor receptor/kinaseCell, 1986
- Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activityNature, 1986
- Further characterization of the glucocorticoid-induced antiphospholipase protein “Renocortin”Biochemical and Biophysical Research Communications, 1983
- A very mild method allowing the encapsulation of very high amounts of macromolecules into very large (1000 nm) unilamellar liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1983