Localization of Type III Procollagen mRNA in Areas of Liver Fibrosis by in situ Hybridization
- 1 November 1989
- journal article
- research article
- Published by Wiley in Acta Pathologica Japonica
- Vol. 39 (11) , 719-724
- https://doi.org/10.1111/j.1440-1827.1989.tb02420.x
Abstract
Localization of type III procollagen mRNA in human liver was studied by in situ hybridization using human 0‐1(111) procollagen cDNA. Frozen and paraformaldehyde‐fixed sections of biopsied human liver from patients with chronic hepatitis and cirrhosis were examined using the digox‐igenin labeled cDNA probe. Localization of the type III procollagen mRNA was demonstrated not only in the cytoplasm of mesenchymal cells but also in a large number of hepatocytes, in proportion to the extent of fibrosis. These results suggest that hepatocytes play an important role in fibrogenesis in the liver.Keywords
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