FURTHER STUDIES ON MOBILIZATION OF CFUs

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Abstract
Mobilization of CFUs [pluripotential stem cells] from [mouse] hemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohemagglutinin (PHA), concanavalin (Con) A, trypsin and DFP-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after i.v. injection. Some variation in activity could not be excluded. As with Salmonella typhosa [S. typhi] LPS, zymosan gave an increase incirculating CFUs during the first few hours and a 2nd peak a few days later. After injection of zymosan an S. typhosa LPS, the 2nd peak in the blood was accompanied by a large increase in CFUs numbers in the spleen. PHA gave an immediate mobilization of CFU, but the mobilization after injection of Con A during the first few hours occurred more slowly. After injection of S. typhosa LPS, zymosan and PHA the blood C3 [complement component 3] level was depressed considerably. The C may be involved in the early mobilization of CFUs. Dexamethasone, a synthetic hormone reported to give sequestration of several cell types in the bone marrow, did not inhibit the early and late mobilization of CFUs which normally occurs after injection of S. typhosa LPS.