Synthesis of Pyridoxine by a Pyridoxal Auxotroph of Escherichia coli
- 1 August 1966
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 92 (2) , 333-+
- https://doi.org/10.1128/jb.92.2.333-337.1966
Abstract
Dempsey, Walter B. (University of Florida, Gainesville). Synthesis of pyridoxine by a pyridoxal auxotroph of Escherichia coli . J. Bacteriol. 92: 333–337. 1966.—A pyridoxal auxotroph of Escherichia coli B produced pyridoxol and pyridoxol 5′-phosphate during starvation for pyridoxal. The identification of these compounds was made both by bioassay and by ion-exchange chromatography. Pyridoxol 5′-phosphate oxidase activity was absent in extracts of the auxotroph. The rate of synthesis of total pyridoxine by a pyridoxal-starved culture of this auxotroph was 6.0 × 10 −6 moles per mg per hr. Cellular content of pyridoxine was constant at 4.0 × 10 −10 moles/mg.This publication has 7 references indexed in Scilit:
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