Thiocyanate Hydrolase Is a Cobalt-Containing Metalloenzyme with a Cysteine-Sulfinic Acid Ligand
- 22 December 2005
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (3) , 728-729
- https://doi.org/10.1021/ja057010q
Abstract
Thiocyanate hydrolase (SCNase) purified from Thiobacillus thioparus THI115 hydrolyzes thiocyanate to carbonyl sulfide and ammonia. DNA sequences of the cloned genes revealed the close relation of SCNase to nitrile hydratase (NHase). The consensus sequences for coordination of the metal ion found in NHases were also conserved in the γ subunit of SCNase. Here, we showed that the SCNase contained one cobalt atom per αβγ heterotrimer. UV−vis absorption spectrum suggested that the cobalt exists as a non-corrin ion. Reduced SCNase showed an ESR signal characteristic of low-spin Co2+, which closely resembled that of the Co-type NHases. Mass spectrometry for the peptide fragment containing the metal-binding motif of the SCNase γ subunit indicated that the cysteine residue at position 131 was post-translationally oxidized to a cysteine-sulfinic acid. From these results, we concluded that SCNases and NHases form a novel non-corrin and/or non-heme protein family having post-translationally modified cysteine ligands.Keywords
This publication has 15 references indexed in Scilit:
- Enzymatic synthesis of acrylamide: a success story not yet overPublished by Elsevier ,2003
- Crystal Structure of Cobalt-Containing Nitrile HydrataseBiochemical and Biophysical Research Communications, 2001
- Arginine 56 mutation in the β subunit of nitrile hydratase: importance of hydrogen bonding to the non-heme iron centerJournal of Inorganic Biochemistry, 2000
- Post‐translational modification is essential for catalytic activity of nitrile hydrataseProtein Science, 2000
- A Stereoselective Cobalt-Containing Nitrile HydrataseBiochemistry, 1997
- Nitrile Hydratase from Rhodococcus rhodochrous J1 Contains a Non-Corrin Cobalt Ion with Two Sulfur LigandsJournal of the American Chemical Society, 1996
- Resonance Raman Spectroscopy of Nitrile Hydratase, a Novel Iron−Sulfur EnzymeBiochemistry, 1996
- A novel iron-sulfur center in nitrile hydratase from Brevibacterium spJournal of the American Chemical Society, 1991
- Nitrile hydratase. The first non-heme iron enzyme with a typical low-spin iron(III)-active centerJournal of the American Chemical Society, 1987
- Characteristics of Thiobacillus thioparus and its thiocyanate assimilationCanadian Journal of Microbiology, 1978