Tryptic Activation of Plasma Renin Activity*
- 1 June 1980
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 50 (6) , 983-988
- https://doi.org/10.1210/jcem-50-6-983
Abstract
Trypsinization of human plasma, like acid activation or cryoactivation, increases renin activity, as determined by subsequent enzymatic assay. In three plasma pools, tryptic activation was maximal within 2 min at a trypsin concentration of 500 μg/ml, decreasing athigher concentrations. Neither prolongation of trypsin exposure for up to 1 h nor temperature (0or 37 C) mattered. In plasmas from 26 healthy volunteers ambulatory for 3 h, trypsin increased PRA3-fold from 2.8 ± 0.4 to 8.3 ± 0.7 ng/ml.h(PJ ClinEndocrinol Metab50: 983, 1980)Keywords
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