Rate and Rate-Determining Step of Hydrogen-Atom-Induced Strand Breakage in Poly(U) in Aqueous Solution under Anoxic Conditions
Open Access
- 1 April 1985
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 40 (3-4) , 247-253
- https://doi.org/10.1515/znc-1985-3-419
Abstract
The rate constant for strand breakage in poly(U) after reaction with H atoms in deoxygenated aqueous solution was determined to be k = 1.5 s-1 at pH = 4-5 and 24.degree. C. Dithiothreitol prevents strand break formation by reacting with H-adduct radicals of poly(U) with a rate constant of 5 .times. 106 M-1 s-1. The rate-determining step in H atom-induced strand breakage in poly(U) at pH .ltoreq. 6 is the decay of uracil moiety H-adduct radicals via H-abstraction from the ribose moiety.Keywords
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