MOUSE MONOCLONAL ANTI‐N: II. PHYSICOCHEMICAL CHARACTERIZATION AND ASSESSMENT FOR ROUTINE BLOOD GROUPING

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Abstract
The precise physicochemical conditions under which 2 examples of mouse monoclonal anti-N can be used as blood grouping reagents were defined. The antibodies belong to the IgG1 and IgG2b subclasses, respectively, and both reacted within discrete ranges of temperature and pH with optimal reactions occurring at 20.degree. C or less and pH 8.5. Under these conditions and at concentrations of 2-3 .mu.g/ml, the antibodies were used, in parallel with conventional polyclonal antisera, to type a series of random [human] blood donors. The results obtained with one of the monoclonal antibodies and the polyclonal reagents were in perfect agreement, and the monoclonal antibody was judged to have considerable potential as an N-grouping reagent.