Human recombinant interleukin 1 beta has no effect on intracellular calcium or on functional responses of human neutrophils.
Open Access
- 15 May 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 138 (10) , 3403-3407
- https://doi.org/10.4049/jimmunol.138.10.3403
Abstract
The effect of human recombinant interleukin 1 beta (rIL 1 beta) on human neutrophils was examined. rIL 1 beta, even at concentrations of 100 ng/ml (100 half-maximal T cell stimulating U/ml) did not change significantly the intracellular free calcium concentration, [Ca++]i, whereas the control stimulus, fmet-leu-phe, significantly elevated [Ca++]i. rIL 1 beta also failed to stimulate production of superoxide, degranulation of lysosomal enzymes, phagocytosis of bacterial particles, chemotaxis, or chemokinesis of human neutrophils. This is substantial evidence that superphysiologic concentrations of interleukin 1 have no direct effect on [Ca++]i, as well as on functional responses of neutrophils.This publication has 1 reference indexed in Scilit:
- Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.Proceedings of the National Academy of Sciences, 1984