Evidence for the Existence of meta and para Directing O-Methyltransferases in Tobacco Cell Cultures
Open Access
- 31 January 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 34 (1-2) , 46-50
- https://doi.org/10.1515/znc-1979-1-212
Abstract
The O-methyltransferase of tobacco [Nicotiana tabacum] cell culture was resolved to its meta and para directing forms by chromatography on DEAE-cellulose. Despite similarities in molecular weights and pI [isoelectric points] values of the 2 forms, however, evidence from pH optima, SH-group inhibitors, methylation ratios, SDS [sodium dodecyl sulfate]-acrylamide gels and mixed substrate experiments indicates the existence of 2 discrete enzymes acting at the meta and para positions of caffeic acid and quercetin, respectively. The latter enzyme was less substrate specific than its meta counterpart.Keywords
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