Influence of Embryonic Dominance and Polarity on Adventitious Shoot Formation from Apple Cotyledons in vitro

Open Access
Publisher Website
Google Scholar
Abstract
In vitro studies indicated that an isolated embryonic axis, or an intact embryo with all or part of its cotyledons, of a ‘Delicious’ apple (Malus Xdomestica Borkh.) produced only a single shoot and root. Adventitious shoot formation was observed when the embryonic axis was excised and the cotyledons were grown in vitro. The number of shoots formed increased as the length of the transverse cut surface was increased. More than 30 shoots per explant were obtained when both the proximal and distal one-third sections of the cotyledon (relative to the embryonic axis) were removed. An increase in shoot number per explant resulted in decreased shoot length. A polar phenomenon was observed in all excision treatments; adventitious shoot formation occurred only at the proximal cut end of the cotyledon.

This publication has 0 references indexed in Scilit: