The influence of muscle-conditioned media on chick embryo brainstem neurons in culture

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Abstract
Brainstem pieces from the trigeminal region of the metencephalic basal plate of 10‐day chick embryos were dissociated and cultured in control conditions or in the presence of muscle‐conditioned medium (MCM). The MCM was derived from age‐matched target tissue relevant to this neuronal region (jaw musculature), from relevant target tissue of an age at which innervation would initially be taking place (4 days), and from nonrelevant target tissue also of an early stage (4‐day limb bud). Neuronal survival and differentiation was assessed daily, for 7 days. Survival and differentiation were significantly enhanced by the 4‐day jaw MCM compared to both the controls and the cultures grown with 10‐day jaw MCM and 4‐day limb MCM. These measures in the presence of 10‐day jaw MCM and 4‐day limb MCM did not differ, but surpassed that seen in control cultures. The results are compared to the more specific responsiveness seen in earlier (2‐day) neural tube cultures, and their relationship to in vivo regenerative nerve fiber outgrowth is considered.