ACTIVATION OF SUPPRESSOR FUNCTION OF HUMAN PERIPHERAL-BLOOD T-CELLS BY (+)CYANIDANOL-3 - ITS APPLICATION TO CHRONIC ACTIVE LIVER-DISEASES

  • 1 January 1983
    • journal article
    • research article
    • Vol. 52  (2) , 430-436
Abstract
(+)Cyanidanol-3, a substance belonging to the flavonoids group, has been used in acute viral hepatitis and chronic active liver disease (CALD). Studies were undertaken to determine if (+)cyanidanol-3 could affect a function of mitogen stimulated peripheral blood lymphocytes (PBL) in humans. (+)Cyanidanol-3 was added directly to pokeweed mitogen (PWM) stimulated PBL or co-culture of B and T cells resulting in severe suppression of Ig production. This suppression was mediated by radiosensitive T cells. When normal T cells pre-incubated with 25 .mu.g/ml of (+)cyanidanol-3 for 48 h were cultured with freshly prepared autologous or allogeneic normal PBL in the presence of PWM, Ig production was markedly suppressed. There was a consistent suppression of blast transformation of concanavalin A stimulated autologous or allogeneic responding PBL by (+)cyanidanol-3 pre-treated normal T cells. (+)Cyanidanol-3-induced suppresor cell activity of T cells from patients with CALD was significantly decreased (P < 0.001) when compared with that of normal individuals. These studies may explain the therapeutic effect of (+)cyanidanol-3 on certain types of liver disease.