Saturation kinetics of coenzyme Q in NADH and succinate oxidation in beef heart mitochondria
- 19 October 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 311 (2) , 107-109
- https://doi.org/10.1016/0014-5793(92)81378-y
Abstract
The saturation kinetics of NADH and succinate oxidation for Coenzyme Q (CoQ) has been re-investigated in pentane-extracted lyophilized beef heart mitochondria reconstituted with exogenous CoQ10. The apparent ‘K m’ for CoQ10 was one order of magnitude lower in succinate cytochrome c reductase than in NADH cytochrome c reductase. The K m value in NADH oxidation approaches the natural CoQ content of beef heart mitochondria, whereas that in succinate oxidation is close to the content of respiratory chain enzymes.Keywords
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