Determination of the O-serovars ofPseudomonas aeruginosa by slide coagglutination

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Abstract
Determination of the somatic (O-) antigens ofPseudomonas aeruginosa by conventional slide agglutination is frequently complicated by the barely discernible, slow reaction of native cells. For diagnostic purposes a more practical procedure, a coagglutination test, has been developed in which protein A bearingStaphylococcus aureus (ATCC 12598) cells are added to the agglutination process occurring between specific anti-O serum and nativePseudomonas aeruginosa. Compared to the conventional method, slide O-coagglutination yields larger agglutinates in a shorter mean reaction time, i.e. one minute vs four minutes. Moreover, strains not reacting in the O-agglutination method or reacting only with polyvalent anti-O serum can be grouped by O-coagglutination, and cross reactions between reference strains of different O-groups do not occur. This method facilitates O-grouping ofPseudomonas aeruginosa in epidemiological investigations.

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