Infrared Spectroscopy of Liver Glycogen in Normal and Irradiated Adult and Fetal Rats.

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Abstract
Dried frozen sections of liver from non-fasted adult and from fetal rats showed a characteristic infrared absorption band at 9.6-9.7 [mu] which is caused by glycogen. The slight variations in location of the band are discussed. Ratios of optical densities between this glycogen band and a "protein band" (6.44 [mu]) were used for semiquantitative estimation of glycogen in liver sections. Relative glycogen content in adult and fetal liver under various nutritional conditions was detd. Spectroscopic examination of liver glycogen after X-ray irradiation showed the existence of an "acute" and "delayed" irradiation effect, the former increasing and the latter decreasing liver glycogen.

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