Effects of Donor Age on Neoplastic Transformation of Adult Mouse Bladder Epithelium In Vitro
- 1 April 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 62 (4) , 1017-1023
- https://doi.org/10.1093/jnci/62.4.1017
Abstract
Neoplastic transformation of C57BL/lcrf-at mouse bladder epithelium was induced in long-term primary cultures by a single 24-hour treatment with 7,12-dimethylbenz[a]anthracene on day 2 of culture. Transformed foci appeared earlier (40–60 days) and at a higher frequency (28%) in cultures from old donors (28–30 mo) compared with 100 days and 0.9% in cultures from young adult donors (5–7 mo). After transplantation into syngeneic mice, transformed cells produced carcinomas. Spontaneous epithelial transformation occurred in dimethyl sulfoxldetreated old cultures after the same interval (40–60 days) as in the carcinogen-treated cultures but at a lower frequency (5.3%). Spontaneous epithelial transformation did not occur in cultures from young donors.Keywords
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