in vitro effects of recombinant human interferon gamma on human mesothelioma cell lines

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Abstract
Malignant mesothelioma is a tumor arising from serous surfaces and often related to asbestos exposure. Malignant mesothelioma is resistant to various forms of therapy. Radiotherapy, surgery or chemotherapy only slightly improve prognosis. IFN‐γ produces complete or partial responses in stage‐l patients with malignant mesothelioma. The in vitro biological effect of IFN‐γ on malignant mesothelioma cells remains poorly elucidated. In the present study, 32 well‐characterized human mesothelioma cell lines (HMCL) were treated with r‐hu IFN‐‐γ at 4 doses and cell growth was determined by a colorimetric method (MTT assay). Among the 32 HMCLs tested, 11 exhibited significant cell‐growth inhibition; 16 were insensitive to r‐hu IFN‐γ, and 5 were slightly inhibited. The sensitive cell lines were strongly inhibited by r‐hu IFN‐γ. Our results show that HMCL exhibit a large range of responses to r‐hu IFN‐γ, some of which can be compared with those obtained in vivo in humans.