Transcriptionally active nuclei isolated from intact bone reflect modified levels of gene expression in skeletal development and pathology
- 1 June 1994
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 55 (2) , 182-189
- https://doi.org/10.1002/jcb.240550205
Abstract
Transcriptional regulation of gene expression in vivo in bone, associated with normal development or skeletal disorders, to date, has not been studied. We report the successful isolation of nuclei that are transcriptionally active from normal and osteopetrotic rat bone. Transcription rates of cell growth and bone-related genes (including histone H4, c-fos, c-jun, TGFβ1, β2 macroglobulin, collagen, fibronectin, osteocalcin, osteopontin, and tartrate resistent acid phosphatase) change as a function of calvarial development from birth to 6 weeks and are selectively modified in osteopetrotic animals. Additionally, nuclei isolated from intact bone yield promoter binding factors. Bone nuclei, which transcribe faithfully and contain the normal complement of nuclear protein factors, offer a powerful approach for investigating in vivo gene regulation in skeletal development and pathology.Keywords
This publication has 33 references indexed in Scilit:
- Glucocorticoids promote development of the osteoblast phenotype by selectively modulating expression of cell growth and differentiation associated genesJournal of Cellular Biochemistry, 1992
- Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cellsJournal of Cellular Physiology, 1990
- Regulation of bone sialoprotein mRNA by steroid hormones.The Journal of cell biology, 1989
- Specific interactions of histone H1 and a 45 kilodalton nuclear protein with a putative matrix attachment site in the distal promoter region of a cell cycle‐regulated human histone geneJournal of Cellular Physiology, 1989
- Tissue specificity and developmental expression of rat osteopontinBiochemical and Biophysical Research Communications, 1987
- A comparative immunocytochemical study on the subcellular distributions of 44 kDa bone phosphoprotein and bone γ-carboxyglutamic acid (Gla)-containing protein in osteoblastsJournal of Bone and Mineral Research, 1987
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- c-fos protein can induce cellular transformation: A novel mechanism of activation of a cellular oncogeneCell, 1984
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Pathogenesis of osteopetrosis in the ia rat: Reduced bone resorption due to reduced osteoclast functionJournal of Anatomy, 1973