Human fibroblasts show expression of the leukotriene‐A4‐hydrolase gene, which is increased after simian‐virus‐40 transformation
- 1 July 1990
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 191 (1) , 27-31
- https://doi.org/10.1111/j.1432-1033.1990.tb19089.x
Abstract
Human fibroblasts in cell culture converted the epoxide intermediate leukotriene A4 into the potent chemotaxin leukotriene B4. The identity of leukotriene B4 was ascertained by its mobility in reverse-phase high performance liquid chromatography, ultraviolet spectroscopy and gas chromatography/mass spectrometry. The presence of the enzyme responsible for the conversion (i.e. leukotriene A4 hydrolase), as well as the corresponding mRNA, were demonstrated by Western and Northern blot analyses. Leukotriene-A4-hydrolase enzyme activity, protein and mRNA were all enhanced (approximately threefold) in human fibroblasts that had been transformed by simian virus 40.This publication has 32 references indexed in Scilit:
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