UPTAKE OF SODIUM INTO RAT ISOLATED SEMINIFEROUS TUBULES IN VITRO

Publisher Website
Google Scholar
Abstract
The rate of 22Na uptake into isolated rat seminiferous tubules has been studied in vitro. The intracellular Na exchanged rapidly with the outside Na+ and within 26 min exposure to 22Na isotopic equilibrium was reached. The relationship between Na+ influx at 2 min and external Na concentrations was linear, suggesting that Na entry into seminiferous tubules is by simple diffusion. The intracellular Na+ concentration as measured by the 22Na taken up into the tubules at equilibrium condition was elevated in the absence of peritubular K+ and Ca2+ and treatment of the tubules with ouabain. No effects of amiloride and acetazolamide on the intracellular Na+ concentration were found. The significance of the 22Na uptake in relation to fluid secretion in the isolated seminiferous tubules was discussed.

This publication has 1 reference indexed in Scilit: