Adenovirus infection targets the cellular protein kinase CK2 and RNA‐activated protein kinase (PKR) into viral inclusions of the cell nucleus

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Abstract
The effects of the adenovirus infection on the distribution of the cellular protein kinase CK2 and double‐stranded RNA‐activated protein kinase (PKR) were examined at the ultrastructural level. Immunogold labeling revealed the redistribution of CK2 subunits and PKR to morphologically distinct structures of the cell nucleus. The electron‐clear amorphous structures, designated pIX nuclear bodies in our previous work (Rosa‐Calatrava et al., 2001), contained CK2 α and PKR. The protein crystals, which result from the regular assembly of hexon, penton base, and fiber proteins [Boulanger et al. (1970) J Gen Virol 6:329–332], contained CK2 β and PKR. Both viral structures were devoid of viral RNA, including the PKR‐inhibitor VA1 RNA generated by the RNA polymerase III. Instead, VA1 RNA accumulated in PKR‐free viral compact rings in which the viral RNA generated by the RNA polymerase II was excluded. Microsc. Res. Tech. 56:465–478, 2002.