NO induces a cGMP‐independent release of cytochrome c from mitochondria which precedes caspase 3 activation in insulin producing RINm5F cells
Open Access
- 5 October 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 459 (2) , 238-243
- https://doi.org/10.1016/s0014-5793(99)01255-7
Abstract
Exposure of RINm5F cells to interleukin‐1β and to several chemical NO donors such as sodium nitroprusside (SNP), SIN‐1 and SNAP induce apoptotic events such as the release of cytochrome c from mitochondria, caspase 3 activation, Bcl‐2 downregulation and DNA fragmentation. SNP exposure led to transient activation of soluble guanylate cyclase (sGC) and prolonged protein kinase G (PKG) activation but apoptotic events were not attenuated by inhibition of the sGC/PKG pathway. Prolonged activation of the cGMP pathway by exposing cells to the dibutyryl analogue of cGMP for 12 h induced both apoptosis and necrosis, a response that was abolished by the PKG inhibitor KT5823. These results suggest that NO‐induced apoptosis in the pancreatic β‐cell line is independent of acute activation of the cGMP pathway.Keywords
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